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|_| \_\ \___| \__| |_| | .__/ \___| \__,_| |_| \__,_|
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VSFP
ββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ
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VSFP (englisch Voltage-sensitive fluorescent proteins) ist eine Gruppe von Fusionsproteinen, die bei Spannung ihre Fluoreszenz Γ€ndern.cite-ref-1[1]cite-ref-2[2]
Contents
β’ Eigenschaften
β’ Anwendungen
β’ Einzelnachweise
ββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ
Eigenschaften
Ein VSFP besteht aus zwei Teilen. Der erste Teil stammt aus einer spannungsabhΓ€ngigen ProteindomΓ€ne aus einer Phosphatase (Ci-VSP) von Ciona intestinalis.cite-ref-3[3] Der zweite Teil besteht aus einem fluoreszierenden Protein wie dem GrΓΌn fluoreszierenden Protein (GFP). Mit unterschiedlichen fluoreszenten Proteinen wurden verschiedene VSFP erzeugt.cite-ref-4[4]
Anwendungen
VSFP werden unter anderem als Redox-abhΓ€ngige Biosensoren und als Reporterproteine verwendet. Alternative optische Methoden verwenden z. B. PROPScite-ref-5[5] oder spannungsabhΓ€ngige Farbstoffe.
Einzelnachweise
cite-note-11. β H. Mutoh, A. Perron, W. Akemann, Y. Iwamoto, T. KnΓΆpfel: Optogenetic monitoring of membrane potentials. In: Experimental Physiology. Band 96, Nummer 1, Januar 2011, S. 13β18, ISSN 1469-445X. doi:10.1113/expphysiol.2010.053942. PMID 20851856.
cite-note-22. β H. Mutoh, W. Akemann, T. KnΓΆpfel: Genetically engineered fluorescent voltage reporters. In: ACS Chemical Neuroscience. Band 3, Nummer 8, August 2012, S. 585β592, ISSN 1948-7193. doi:10.1021/cn300041b. PMID 22896802. PMC 3419450 (freier Volltext).